Skip to content
  • +91 7977502315
  • Ghatkopar, Mumbai
  • info@amodachem.com
  • Home
  • About Us
  • Products
    • Biological Wastewater Treatment
    • MLSS BOOSTER for Wastewater Treatment in India
    • Wastewater Treatment Chemicals
    • Industrial Chemicals
  • Industries Served
  • Blogs
  • Clients
  • Contacts
  • Home
  • About Us
  • Products
    • Biological Wastewater Treatment
    • MLSS BOOSTER for Wastewater Treatment in India
    • Wastewater Treatment Chemicals
    • Industrial Chemicals
  • Industries Served
  • Blogs
  • Clients
  • Contacts
Enquire Now

Understanding MLSS: A Key Parameter in Biological Wastewater Treatment

MLSS in biological wastewater treatment process for STP and ETP systems
  • May 12, 2025

Biological wastewater treatment provides effective wastewater management to ensure discharged water is both safe and free of contaminants.

The most important of the parameters involved is the mixed liquor-suspended solids (MLSS), which is used to express the concentration of biomass in the aeration tank. MLSS is imperative in the activated sludge since it influences pollutant degradation, sludge settling, and treatment efficiency.

Proper MLSS management is essential for optimizing MLSS in wastewater treatment, balancing aeration needs, reducing operational costs, and ensuring compliance with environmental regulations.

Keeping the right MLSS levels boosts microbial activity, stabilizes sludge, and prevents system failures. Poor MLSS control can cause inefficiencies, excess sludge, and regulatory issues, leading to higher costs and environmental risks.

What is MLSS?

MLSS, also known as Mixed Liquor Suspended Solids, is the total concentration of suspended solids in the aeration tank of a biological wastewater treatment plant. It includes organic and inorganic materials, primarily microorganisms that degrade organic pollutants in wastewater.

Components of MLSS

MLSS is composed of:

Microorganisms (biomass): Organisms such as bacteria, protozoa, and fungi which are responsible for decomposing organic matter.

  1. Organic matter: Decomposed fractions of wastewater materials.
  2. Inorganic solids: Materials that are non-biodegradable like silt and minerals.
  3. Keeping these components balanced is key to effective wastewater treatment and pollutant removal.

Keeping these components balanced is key to effective wastewater treatment and pollutant removal.

Importance of MLSS in Wastewater Treatment

Indicator of Treatment Efficiency

Mixed Liquor Suspended Solids (MLSS) is a key measure of the concentration of microorganisms and suspended solids in aeration tanks. It helps operators determine if the biological process effectively breaks down organic matter. Maintaining the right MLSS level ensures optimal treatment performance.

Maintains Biological Stability

A balanced MLSS in wastewater treatment provides a stable microbial population that can effectively break down organic contaminants. When MLSS is too low, microbial activity will be inadequate. Too high can cause process inefficiencies, e.g., sludge bulking.

Affects Sludge Settling and Quality

MLSS concentration in wastewater treatment affects sludge settling in clarifiers. The right levels ensure better sedimentation, prevent sludge carryover, and keep the water clear, helping meet regulations.

Optimizes Aeration Efficiency

Aeration is an energy-intensive process in wastewater treatment. Oxygen transfer efficiency is affected by the concentration of MLSS. Excessive MLSS increases oxygen demand and energy consumption. The proper MLSS concentration maximizes aeration and minimizes operating costs.

Enhances Nutrient Removal

MLSS is essential for removing nitrogen and phosphorus in wastewater treatment. The right concentration helps beneficial bacteria thrive, preventing water pollution and eutrophication.

Ensures Regulatory Compliance

Wastewater treatment plants must follow strict discharge standards for suspended solids and BOD. Proper MLSS control ensures effective treatment, meets regulations and prevents fines.

MLSS in Different Wastewater Treatment Stages

  1. Primary Treatment
  • MLSS does not play a substantial role in this stage.
  • Large solids and debris are removed through screening and sedimentation.
  1. Secondary Treatment (Activated Sludge Process)
  • MLSS is crucial in this biological treatment phase.
  • Bacteria and other microbes digest organic matter, reducing pollution.

Proper aeration and Sludge Retention Time (SRT) are essential for MLSS balance.

  1. Tertiary Treatment
  • While MLSS is managed in the secondary phase, its impact extends to nutrient removal, disinfection, and membrane filtration in tertiary treatment

Factors Affecting MLSS Concentration

Several factors influence MLSS concentration in wastewater treatment plants. Understanding these variables is crucial for maintaining optimal treatment efficiency. 

1. Organic Loading Rate 

  • The amount of organic material entering the system affects MLSS levels. 
  • High organic loading promotes microbial growth, increasing MLSS. 
  • Low organic loading can lead to biomass decay, reducing MLSS. 

2. Hydraulic Retention Time (HRT) 

  • HRT represents the time wastewater remains in the treatment tank. 
  • Longer HRT allows for better microbial activity, stabilizing MLSS levels. 
  • Short HRT may cause biomass washout, leading to low MLSS. 

3. Sludge Wasting Rate 

  • Regular sludge wasting is essential for maintaining appropriate MLSS. 
  • Excessive wasting lowers MLSS, while insufficient wasting leads to excessive biomass buildup. 

4. Aeration Efficiency 

  • Proper aeration ensures microbial activity and prevents sludge-settling issues. 
  • Poor aeration results in low dissolved oxygen levels, affecting microbial metabolism and MLSS stability. 

5. Seasonal Variations 

  • Temperature changes impact microbial activity and MLSS levels. 
  • Cold temperatures slow down microbial growth, reducing MLSS. 
  • Warmer temperatures enhance biological activity, increasing MLSS. 

Optimal MLSS Range for Wastewater Treatment Plants 

Maintaining MLSS within the optimal range is essential for stable treatment performance. The recommended MLSS concentration depends on the specific treatment process: 

Treatment Process Optimal MLSS (mg/L) 
Conventional Activated Sludge 2,000 – 4,000 
Extended Aeration 3,000 – 6,000 
Sequencing Batch Reactor (SBR) 3,000 – 5,000 
Membrane Bioreactor (MBR) 8,000 – 15,000 

Summing Up  

MLSS in wastewater treatment is an essential parameter that decides biological treatment efficiency, sludge stability, and the quality of the effluent. Determination of the factors governing MLSS concentration and the application of right control measures guarantee efficient wastewater treatment. 

At Amoda Chem, we understand the critical role of MLSS in wastewater treatment. Our MLSS booster solutions help optimize microbial activity, improve sludge settling, and enhance treatment efficiency. Partner with us to achieve sustainable, cost-effective wastewater management.  

Struggling with MLSS management? Let our experts help!  

FAQ’s 

  1. Why is MLSS important in biological wastewater treatment? 

Mixed Liquor Suspended Solids (MLSS) is an important parameter in MLSS wastewater treatment. It indicates the amount of microorganisms present in the aeration tank, which decomposes waste. Maintaining the proper MLSS in wastewater treatment enhances system efficiency, minimizes sludge accumulation, and provides cleaner water output.  

  1. What is the ideal MLSS range for wastewater treatment? 

The ideal MLSS in wastewater treatment is generally in the range 2000 – 5000 mg/L based on the process and nature of wastewater. Treatment will be ineffective if the MLSS is below the required level, while higher levels will cause settling problems, high oxygen demand, and sludge bulking.  

  1. Does MLSS affect greenhouse gas emissions? 

If MLSS levels are not managed properly, it can lead to inefficient aeration and higher CO₂ and N₂O emissions. Keeping MLSS at the right level helps save energy, lower the carbon footprint, and improve wastewater treatment performance. 

  1. Does MLSS play a role in energy-efficient wastewater treatment? 

Effective MLSS wastewater treatment requires maintaining optimal MLSS levels to balance microbial activity and aeration efficiency.  

Optimal MLSS management decreases the cost of energy, enhances the use of oxygen, and lessens the costs of handling sludge, and thus makes the treatment more sustainable and economical.  

  1. What is the difference between MLSS and MLVSS? 

MLSS includes organic and inorganic suspended solids, while MLVSS (Mixed Liquor Volatile Suspended Solids) represents the organic, biodegradable fraction that consists mostly of active microorganisms. 

Recent Posts

Understanding MLSS: A Key Parameter in Biological Wastewater Treatment

Read More »

How Primary Effluent Treatment Chemicals Affect MLSS in Effluent Treatment Plants

Read More »

How to Improve COD Reduction with Proper pH Management in Wastewater

Read More »

To Explore More

Click here
At Amoda Chem & Services LLP, we are proud to be one of the leading wastewater treatment chemicals manufacturers in India.

Quick Links

  • Home
  • About Us
  • Products
  • Blogs
  • Contact Us

Our Products

  • Biological Wastewater Treatment
  • MLSS BOOSTER for Wastewater Treatment in India
  • Wastewater Treatment Chemicals
  • Industrial Chemicals

Keep In Touch

  • A-106, Kailas Plaza, Vallabh Baug Extension, Ghatkopar East,Mumbai - 400 077.
  • info@amodachem.com
  • +91 7977502315
Copyright 2025 © All Right Reserved | Design & Developed by
  • Facebook
Back To Top